LumiSens optical DO sensor

Traditional polarographic DO2 sensors need to be polarized for a longer period before they can be used in a process. The Applikon Lumisens sensor can be used right away. The optical technology allows accurate measurements especially in the low oxygen concentration range where classic probes are less accurate. The Lumisens sensor is currently available for our bioreactor range from 250 ml up to 3 liter total volume. The unique design of the smart sensor separates the electronics from the glass sensor part, guaranteeing a long sensor life with repeated sterilizations. Strong magnets keep the electronics in place on the sensor in order to guarantee reproducible positioning and accurate measurements. The integrated health monitor of the sensor shows the status of the fluorphor sensor tip and indicates when the sensor tip needs to be replaced.

LumiSens optical DO sensor

Features

  • Longer sensor life time since sensor is not sterilized
  • Direct use after autoclaving as no polarization is needed
  • Reduced maintenance costs since no electrolyte and membranes have to be replaced
  • Accurate measurements in low oxygen range
  • Long term stability
  • Health status monitor to show sensor condition
  • Insensitive to CO2 fouling
  • Ø8 mm sensor for accurate reading on small scale
  • M12 thread integrated for direct mounting in top plate
  • No interference with biotech-molecules, like mCherry, Texas Red and Chlorophyll a & b due to RedFlash technology

How the LumiSens works

The measurement principle is based on the phase shift between excitation and emission, which is directly related to the partial pressure of oxygen. The RedFlash technology is characterized by high precision and reliability, low cross-sensitivity and fast response. The red light excitation significantly reduces interferences caused by auto fluorescence and reduces stress in biological systems. Besides, due to the excellent luminescence brightness of the RedFlash indicator, the actual sensor matrix can be prepared much thinner, leading to fast response time of the sensor.

LumiSens optical DO sensor
LumiSens optical DO sensor
Facts and Figures

Applications:

  • Mini bioreactors, thanks to its 8 mm diameter
  • 2, 3 & 5 liter autoclavables
  • Long-term processes
  • Anaerobic and micro-aerobic processes
  • Microbial and cell culture processes

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