ez2-Control bundle

Our Applikon BioBundles are complete bioreactor systems with everything you need for successful cultivation.

The turnkey solutions consisting of a controller and a bioreactor include silicone tubing, sample bottles, a spares kit, and Applikon’s data acquisition software, Lucullus Lite.

The bioreactors come in a wide range of volumes: 2L, 3L, 5L, 7L, 15L, and 20L, and they are perfect for cell and microbial cultures. The control system for these small-scale bioreactors is the Applikon ez2-Control.

Customize your bioreactor system with one or more of our add-on packs.

Features

Quality without compromise: we use the highest quality materials.
Ease of use and sophistication in one BioBundle.
Our solutions are configurable and customizable.
Get peace of mind with 1 Applikon ez2-Control per bioreactor.
Acquire data with the included software.
Save costs and time with standardization.

Efficiently Optimized

Applikon bioreactor systems are designed to meet your needs with the utmost reliability and accuracy. Everything is manufactured to operate efficiently, and no detail is overlooked.

A quiet agitation motor is included.
Smooth stainless steel contact surfaces avoid cross-contamination.
The ez2-Control has a sophisticated but straightforward integrated touch screen.
Software updates are easily performed through USB without an external Service Engineer.

Specifications of ez2-Control bundles

Our standard setups are highly sophisticated and can be adapted based on your application. The cell culture and microbial fermentation bundles share many of its components. Nevertheless, there are some differences between the bundles which can be further customized to meet your specific needs.

	Cell Culture	Microbial
Agitator	Lipseal or magnetic with Marine impeller	Lipseal or magnetic with Rushton impellers
Aeration	Air and Oxygen supply via sparger	Air and Oxygen supply via sparger and overlay
pH	Control via liquid alkali pump and CO2 gas supply	Control via liquid alkali and acid addition pump
Temperature	Heating via heating blanket, Peltier optional	Heating via heating blanket, cooling by cold water in heat exchanger
Dissolved Oxygen	Control via Air and O2 gas supply	Control via Air and O2 gas supply and agitation speed
Foam	Optional	Control via anti-foam addition pump
Liquid additions	3 ports in triple inlet and one inoculum port	3 ports in triple inlet, one inoculum port and one septum port
	One liquid addition bottle included	Two liquid addition bottles included

Single and jacketed wall
Total Volume	2 L, 3 L, 5 L, 7 L, 15 L, 20 L
Working Volume	1.7 L, 2.7 L, 3.4 L, 5.4 L, 12 L, 16 L

Drive system	Direct drive, lip sealed or magnetically coupled
Stirrer speed (rpm)	Standard range is 50 – 1250
	2 and 3 liter systems can be supplied with 2000 rpm motor
Impellers	Rushton and marine with outside diameters 45 mm, 60 mm 75 mm or 85 mm

Gas sparger	Porous sparger or L-type sparger
Gas overlay	Yes
Exhaust gas	Water cooled exhaust gas condenser

Sampling	Fixed height or height adjustable sample pipe with optional sampling system
	Sample pipe internal diameters choices are: 1.7 mm, 4 mm, 6 mm or 10 mm
Draining	Drain pipe
Additions	Triple or single inlet ports and optional micro liquid injectors
Optional inlets	Septum, chemostat tube, liquid entry system

pH	Measurement: 12 mm classic pH sensor Control: via acid pump or CO2 gas (rotameter or MFC) in combination with alkali pump
DO2	Measurement: 12 mm classic polarographic DO2 sensor or LumiSens for 2-5 L Control: via a combination of N2, Air, O2 (Rotameter or MFC) and agitation
Temperature	Measurement: Pt-100 sensor in thermowell in topplate Control: cooling and/or heating jacket via bioreactor wall or via internal heat exchanger
Foam	Measurement: Height adjustable conductivity based foam sensor Control: anti-foam addition pump
Level	Measurement: Height adjustable conductivity based level sensor Control: pump for liquid addition or removal
Optional sensors	Biomass, Optical Density, O2 and CO2 off gas, weight balance

Lucullus Lite is included with all BioBundles.

Other software solutions like Standard/Advanced or Expert versions of Lucullus or the BioXpert W10 software can alternatively be supplied with the system.

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