Applications
Unlocking Innovation in Biotechnology: The Baculovirus Expression Vector System (BEVS) with Insect Cells
The Baculovirus Expression Vector System (BEVS) is a sophisticated platform designed to express complex proteins in insect cells. By utilizing a baculovirus—a virus that naturally infects insects—as a vector to introduce genetic material into insect cells, BEVS utilizes the robust protein production machinery of these cells to efficiently produce recombinant proteins.
High Protein Yield:
BEVS is known for its ability to produce large quantities of recombinant proteins. This makes it ideal for applications requiring substantial protein amounts, such as vaccine development, enzyme production, and pharmaceutical research.
Complex Post-Translational Modifications:
Insect cells can perform complex post-translational modifications similar to those in mammalian cells, ensuring that the expressed proteins are properly folded and functional. This is critical for the biological activity and efficacy of many therapeutic proteins.
Scalability:
From small-scale laboratory research to large-scale industrial production, BEVS with insect cells is highly scalable. The system can be easily adapted to various production volumes, providing flexibility to meet different project needs.
Cost-Effective:
Insect cell cultures are generally less expensive to maintain than mammalian cell cultures. This cost-effectiveness, combined with high protein yields, makes BEVS an economical choice for many biotechnology applications.
The process of insect cell cultivation with BEVS
Initial Preparation and Inoculation
The cultivation process begins with careful preparation of the Applikon Autoclavable Bioreactor, focusing on sterility and the precise setup required for optimal insect cell culture. SF9 insect cells, sourced from the fall armyworm (Spodoptera frugiperda), are known for their robustness and are inoculated under aseptic conditions. These cells, when used in conjunction with the baculoviral expression (BEVS) system, are a preferred choice for their high efficiency in recombinant protein production. The BEVS is particularly effective as it utilizes recombinant baculoviruses to introduce DNA into the host cells, promoting overexpression of target proteins in a system that mimics the natural virus-host interaction.
Optimizing Cell Culture Conditions
Creating an environment that closely mimics the natural conditions of insect cells is crucial for their survival and productivity. The Applikon Autoclavable Bioreactor manages key parameters such as:
- Temperature Control: Insect cells are cultured at an optimal temperature of 27°C, critical for promoting robust cell growth and efficient protein synthesis.
- pH and Dissolved Oxygen Management: The bioreactor maintains a pH range of 6.0 to 6.2 and moderate oxygen levels, essential for sustaining cell metabolism and health.
- Gentle Agitation: Employing a gentle agitation system minimizes shear stress, which is particularly essential to insect cells, ensuring even distribution of cells and nutrients.
Nutrient Supply and Metabolic Waste Management
The Applikon Autoclavable Bioreactor is equipped with advanced systems to supply essential nutrients while concurrently managing the removal of metabolic waste. Maintaining this balance is vital to prevent the accumulation of toxic byproducts that could hinder cell growth and productivity.
The advanced system leverages the unique capabilities of insect cell cultures
These and more are the reasons why Applikon glass bioreactors are the ideal choice for cultivating insect cells. Available in different volumes, scale-up is very easy. Cultivating insect cells in the Applikon Autoclavable Bioreactor is a sophisticated method employed in the field of biotechnological innovation. The bioreactor system facilitates the production of viral vectors, vaccines, and recombinant proteins by leveraging the specialized capabilities of the insect cell culture. The bioreactor’s controlled conditions—precise temperature, pH regulation, and gentle agitation—create an ideal environment for maximizing insect cell productivity and quality. This setup supports optimal viral infection and protein expression, improving both yield and quality of the bioproducts.
Advantages of Using the Applikon Autoclavable Bioreactor for Insect Cell Culture
- Precise Environmental Control: Ensures optimal conditions for insect cell growth and recombinant protein production.
- Scalability: Its design allows for easy scale-up from research to production scales, facilitating the transition of bioprocesses from the laboratory to commercial production.
- Cell Culture Shear Protection: Incorporates gentle mixing and oxygenation strategies that protect delicate insect cells from shear stress, enhancing cell viability and productivity.
- Efficiency and Productivity: Through cell culture engineering, it maximizes protein yield and quality, making it an invaluable tool for biopharmaceutical production.
The Applikon Autoclavable Bioreactor is a key element for the cultivation of insect cells. It embodies the integration of cell culture engineering principles with bioreactor technology to provide a controlled, efficient, and scalable platform for producing valuable bioproducts, positioning it as a critical asset in the biotechnological and pharmaceutical industries. Contact us for more information and consulting.
Feautured Products
my-Control bundle
The myControl Bundle consists of our small scale MiniBio bioreactors (250mL, 500mL and 1000mL), the myControl bioprocess controller, data acquisition software and the start-up kit. This space-optimized solution is the perfect partner for your research and development in the laboratory.
This versatile bioprocessing system can be used for both cell cultures as well as microbial cultures.
AppliFlex ST bundle
The AppliFlex ST bundle comes with the 3D printed single-use bioreactor AppliFlex ST and an Applikon bioprocess controller. The bioreactor can be adapted to the process requirements and is perfectly scalable. The reactor comes pre-sterilized and thus reduces the risk of contamination. The system simplifies process preparation and increases throughput.
The AppliFlex ST Bundle is available in 500mL, 3 L and 15L total volume bioreactors for cell cultures or for microbial cultures.
Glass autoclavable bioreactors
The Applikon autoclavable bioreactor is a very popular bioreactor. It is available in 2, 3, 5, 7, 15 and 20-litre volumes. It is easy to adapt if your research changes. Thanks to the modularity and flexibility of the glass bioreactors, you can modify the system to fit any adaptations in process demands.
The glass bioreactors help support and optimise your research and process development. The systems are suitable for both cell culture and microbial culture applications.
Livit Flex bioprocess control system
The Livit Flex bioprocess controller is an intuitive and easily configurable bioprocess controller that fits any biotech upstream R&D application. Livit Flex can be configured as a single or dual control system for single-use or multi-use bioreactors to optimize bench space in the laboratory.
Livit Flex is ideal for use with single-use and reusable bioreactors up to 20L as well as our single-use pilot reactors.
SUPR single-use bioreactor
The SUPR single-use production bioreactor impresses with its simple, scalable and customer-friendly design. The cell culture reactor enables a seamless process transfer from R&D to production.
The SUPR bioreactor is available in the sizes 50L, 250L and 1000L.
BioSep perfusion device
The Applikon BioSep System is a unique cell retention system for perfusion processes. By using high-frequency ultrasonic resonance waves for cell separation instead of a physical membrane, the system offers all the advantages of conventional devices, but without their typical limitations.
The Biosep system is mainly used for cell cultures and yeasts, and plant cells.
Stainless steel bioreactors
Stainless steel bioreactors play a key role in the development and production biotech products. From bench to pilot to full production – the Applikon bioreactors simplify your scale-up through consistent design and scalable control solutions.
Our portfolio offers both standard systems for fermentation and cell cultures as well as customized systems.
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